晚期非小细胞肺癌患者外周血DCreg表达意义及与中医辨证虚实属性差异性研究OA
Significance of DCreg Expression in Peripheral Blood of Patients with Advanced Non-Small Cell Lung Cancer and Its Differences in the Deficiency and Excess Attributes of Traditional Chinese Medicine Syndrome Differentiation
目的 检测调节性树突状细胞(DCreg)在晚期非小细胞肺癌(NSCLC)患者外周血中的表达水平,探索 DCreg 对判断患者病情及虚实证候的临床价值.方法 选取 50 例晚期 NSCLC 患者作为实验组,10 例健康体检者作为对照组.采用流式细胞术检测两组外周血中 DCreg 含量,比较 NSCLC 患者及健康人外周血 DCreg 含量的差异,NSCLC 患者外周血 DCreg 在不同临床特征组别间的差异,并分析 DCreg 含量与 T 细胞亚群、血清细胞因子的相关性.此外,比较 DCreg 含量在不同虚实证型之间的差异,并通过多分类 Logistic 回归分析探讨对 NSCLC 患者虚实证候预测效果最好的指标联合方案.结果 NSCLC 患者外周血 DCreg 含量明显高于健康人外周血 DCreg 含量(P<0.01).DCreg 可能与吸烟史、临床分期、Ki-67、Karnofsky 评分、CD3+T、调节性 T 细胞(Treg)、白细胞介素(IL)-2、干扰素(IFN)-γ、IL-4、IL-10 存在一定关系.虚证组NSCLC 患者外周血DCreg 含量明显高于实证组(P<0.01);虚实夹杂证组 DCreg 含量高于实证组(P<0.05).相对于实证,DCreg 含量高、Treg 含量高,且 Karnofsky 评分<80 的患者辨证为虚证的可能性更高.结论 DCreg 可能成为评价 NSCLC 患者病情的潜在指标,DCreg 的检测对于中医虚实证型的判定具有一定的参考价值,在临床实际应用中尚需结合 Treg 含量以及 Karnofsky 评分等指标综合判断.
Objective To detect the expression level of regulatory dendritic cells(DCreg)in the peripheral blood of patients with advanced non-small cell lung cancer(NSCLC)and to explore the clinical value of DCreg in assessing patient condition and deficiency-excess syndrome differentiation.Methods Fifty patients with advanced NSCLC were enrolled as the experimental group,and 10 healthy individuals were included as the control group.Flow cytometry was used to measure DCreg levels in the peripheral blood of both groups.Differences in DCreg levels between NSCLC patients and healthy controls were compared,as well as among NSCLC patient subgroups with different clinical characteristics.Correlations between DCreg levels and T cell subsets,as well as serum cytokines,were analyzed.In addition,differences in DCreg levels among different deficiency-excess syndrome types were compared,and multinomial logistic regression analysis was used to identify the optimal combination of indicators for predicting deficiency and excess syndromes in NSCLC patients.Results DCreg levels in the peripheral blood of NSCLC patients were significantly higher than those in healthy controls(P<0.01).DCreg levels showed correlations with smoking history,clinical stage,Ki-67,Karnofsky score,CD3+T cells,regulatory T cells(Treg),interleukin(IL)-2,interferon(IFN)-γ,IL-4,and IL-10.DCreg levels in the deficiency syndrome group were significantly higher than those in the excess syndrome group(P<0.01).DCreg levels in the mixed deficiency-excess syndrome group were higher than those in the excess syndrome group(P<0.05).Compared with excess syndrome,patients with high DCreg levels,high Treg levels,and a Karnofsky score<80 were more likely to be classified as having deficiency syndrome.Conclusion DCreg may serve as a potential biomarker for evaluating the condition of NSCLC patients.The detection of DCreg provides valuable reference for identifying deficiency and excess syndromes in TCM,though comprehensive assessment incorporating indicators such as Treg levels and Karnofsky score is recommended for clinical application.
熊宏泰;马苏苏;宋英;蔡柳;郑红刚
中国中医科学院广安门医院,北京 100053中国中医科学院广安门医院,北京 100053中国中医科学院广安门医院,北京 100053陕西中医药大学,陕西 咸阳 712046中国中医科学院广安门医院,北京 100053
医药卫生
非小细胞肺癌调节性树突状细胞免疫功能辨证论治虚证实证
Non-small cell lung cancerRegulatory dendritic cellsImmune functionSyndrome differentiation and treatmentDeficiency syndromeExcess syndrome
《中国中医基础医学杂志》 2026 (4)
874-881,8
国家自然科学基金面上项目(82174463)中央高水平中医医院临床研究和成果转化能力提升项目(HLCMHPP2023085)
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