VEGF基因多态性与系统性红斑狼疮的遗传易感性研究OA

Objective:To analyze the relation between gene single nucleotide polymorphisms(SNPs)of vascular endothelial growth factor(VEGF)and genetic susceptibility of neuropsychiatric systemic lupus erythematosus(NPSLE)based on NPSLE pathogenesis.Methods:A total of 82 patients without other autoimmune diseases who were confirmed as NPSLE at The First Affiliated Hospital of Guangxi Medical University and The People's Hospital of Guangxi Zhuang Autonomous Region from August 2020 to December 2020 were enrolled as NPSLE group.Meanwhile,166 patients with systemic lupus erythematosus(SLE)but without neuropsychiatric symptom were included as non-NPSLE group.According to the principle of matching age with patients of positive control group,150 healthy individuals from the Physical Examination Center of The People's Hospital of Guangxi Zhuang Autonomous Region were selected as the healthy control group.The allele and genotype of 3 SNP of subjects in each group were retrospectively analyzed.Then,the correlation of the risk of occurring disease between NPSLE and non-NPSLE was further analyzed.Results:The frequencies of the genotype of rs699947 locus(AA,AC and CC)of NPSLE group were respectively 11%,64.6%and 24.4%,while those of non-NPSLE group were respectively 4.8%,80.7%and 14.5%,and the differences of them between two groups were significant(x2=7.971,P=0.019).The difference of the frequencies of G and A allele at rs10434 locus between NPSLE group and healthy control group was significant(x2=12.564,P<0.05).The difference of the frequency of the genotypes(GG,GA and AA)between NPSLE group and healthy control group was significant(x2=25.284,P<0.05).The difference of the frequency of the genotypes(GG,GA and AA)between NPSLE group and non-NPSLE group was significant(x2=7.653,P=0.022).The GA and A allele of mutant heterozygous mode,and dominant gene mode(GA+AA)appeared correlation with occurring NPSLE(OR=5.069,95%CI:2.565-10.020,OR=1.990,95%CI:1.331-2.975,OR=4.867,95%CI:2.474-9.573),respectively.The difference of the frequency of rs833070 genotype(GG,GA,AA)between non-NPSLE group and healthy control group was significant(x2=6.080,P=0.048).Conclusion:It is possible that there is correlation between AC genotype of rs699947 and risk of occurring non-NPSLE disease.The A allele,mutant heterozygous GA,and dominant gene(GA+AA)of VEGF rs10434 are correlation with the risk of occurring NPSLE disease.The A allele and dominant mode(GA+AA)are also correlation with the risk of occurring non-NPSLE disease.There is not significant correlations between rs833070 genetic polymorphism and the risk of occurring NPSLE and non-NPSLE(P>0.05).