基于磁共振多模态功能成像的静脉源性脑微出血小鼠影像表征研究OA

[Objective]Cerebral microbleeds(CMBs)are insidious in onset and have a complex mechanism of occurrence.Therefore,this study aims to explore whether there is a causal relationship between them and CMBs by preparing models of venous circulation disorders at different positions,and to evaluate the detection efficiency of different MRI functional sequences for venous CMBs.[Methods]Twenty C57BL/6J mice were selected,and the internal and external jugular veins were selectively ligated to prepare four groups of mouse models of cerebral venous circulation disorders at different locations(n=5),namely the bilateral internal and external jugular vein ligation(JVL),bilateral external jugular vein ligation(EJV),bilateral internal jugular vein ligation(IJV),and control groups.Subsequently,MRI multimodal functional imaging,and pathological HE staining were carried out for comprehensive evaluation.[Results]Magnetic resonance multimodal functional imaging showed that CMBs appeared in the SWI sequence in the JVL group of mice,and there were no abnormalities in the brains of mice in the other groups.According to the pathological results,CMBs occurred in both the bilateral IJV group and the JVL group of mice,and the number and area of CMBs in the latter were larger(P<0.05).Spearman correlation analysis showed that the number of CMBs in a single slice(r=0.82,P<0.01)and the area of CMBs(r=0.97,P<0.001)were positively correlated with the number of CMBs detected by SWI.[Conclusion]Cerebral venous circulation disorders can lead to the occurrence of CMBs,and circulation disorders in different parts have different pathogenicity for CMBs.For venous origin CMBs,susceptibility weighted imaging has higher sensitivity compared with T1WI,T2WI,and DWI imaging methods.