诃子提取物调控COX2/PGE2信号通路对子宫肌瘤细胞生物学行为的影响OA

Objective To investigate the effects and mechanisms of different concentrations of Medicinal Terminal Fruit extracts on the biological behavior of uterine fibroid cells.Methods Human uterine fibroid cells were assigned into control group,low-,medium-,and high-concentration(60,90,and 120 μg/mL)Medicinal Terminal Fruit extract groups,and high-concentration Medicinal Terminal Fruit extract+LPS group(120 μg/mL Medicinal Terminal Fruit extract and 3 μg/mL LPS).The CCK-8 assay was used to detect the proliferative ability of uterine fibroid cells in each group.Flow cytometry was used to detect the apoptosis rate of uterine fibroid cells in each group.The scratch test was used to detect the migratory ability of the uterine fibroid cells in each group.Transwells were used to detect the invasive ability of the uterine fibroid cells in various groups.Western blotting was performed to detect the expression levels of cyclooxygenase-2(COX2)and prostaglandin E2(PGE2)proteins in uterine fibroid cells from various groups.Results Compared with the control group,the proliferation rate,migration rate,and invasive abilities of uterine fibroid cells in each of the Medicinal Terminal Fruit extract groups were significantly reduced(P<0.05),the apoptosis rate was significantly increased(P<0.05),and the expression of COX2 and PGE2 proteins in uterine fibroid cells were significantly decreased(P<0.05).Medicinal Terminal Fruit extract inhibited the malignant behavior of uterine fibroid cells,and this effect increased with increasing extract concentration(P<0.05).However,COX2 activator LPS reversed the effect of Medicinal Terminal Fruit extract on uterine fibroid cells(P<0.05).Conclusion Medicinal Terminal Fruit extract reduced the proliferation,migration,and invasive abilities of uterine fibroid cells and promoted apoptosis.This effect may be achieved by inhibition of the COX2/PGE2 signaling pathway.