LINC01082通过调节miR-18a-3p/CADM2轴抑制食管鳞状细胞癌细胞增殖、迁移和侵袭OA

Objective To investigate the effects of LINC01082 on the proliferation,migration,and invasion of esophageal squamous cell carcinoma(ESCC)cells by regulating the miR-18a-3p/CADM2 axis.Methods LINC01082,miR-18a-3p,and CADM2 expressions in ESCC tissues and adjacent normal tissues were analyzed using the UALCAN database.LINC01082,miR-18a-3p,and CADM2 mRNA expressions in ESCC cells and normal esophageal epithelial cells were measured using real-time quantitative PCR.LINC01082 was over-expressed in the ESCC cell line KYSE150,and its effects on the proliferation,migration,and invasion of KYSE150 cells were detected using the cell counting kit(CCK)-8,plate colony formation,scratch,and Transwell assays.A dual luciferase reporter assay was performed to detect the interactions between LINC01082 and miR-18a-3p and between miR-18a-3p and CADM2.Results Compared with those in the adjacent normal tissues,LINC01082 and CADM2 expression levels were significantly lower in ESCC tissues,whereas the miR-18a-3p expression level was significantly higher(P<0.05).Compared with normal esophageal epithelial cells(SHEE cells),LINC01082 and CADM2 mRNA expression levels were significantly decreased in KYSE150 cells,whereas miR-18a-3p expression level was significantly increased(P<0.05).Notably,LINC01082 overexpression significantly reduced cell proliferation,migration,and invasion abilities of KYSE150 cells.The results of dual luciferase reporter assay revealed a targeting interaction between LINC01082 and miR-18a-3p,as well as between miR-18a-3p and CADM2.Conclusion LINC01082 overexpression may inhibit the proliferation,migration,and invasion of ESCC cells by downregulating miR-18a-3p and promoting CADM2 expression.