链霉菌细胞色素P450酶的功能及改造和应用OA

Cytochrome P450 enzymes represent a class of heme-containing protease widely distributed across the biological kingdom.Endowed with a broad substrate scope,diverse catalytic reaction profiles,high regio-and stereoselectivity toward substrates,and mild catalytic conditions,these enzymes stand out as superior biocatalysts with good potential of application.As one of the primary producers of natural products,Streptomyces harbors a wealth of P450 enzymes and natural product biosynthetic gene clusters(BGCs)in its genome,making it a versatile treasure trove for the generation of bioactive small-molecule compounds.This review summarizes the catalytic reactions by Streptomyces-derived P450 enzymes in natural product biosynthesis and delineates the engineering strategies for addressing the practical application challenges of P450 enzymes,aiming to provide some reference and guidance for in-depth research on P450 enzymes and their applications.