灵芝胞外粗多糖对常温与高温环境下白灵菇菌丝生长的影响OA
Effects of Ganoderma lucidum crude exopolysaccharide on the mycelial growth of Pleurotus tuoliensis under normal and high temperature
为缩短白灵菇生长周期同时缓解高温对白灵菇菌丝生长带来的负面效应,以泰山赤芝为试材,采用液体发酵法(70%、85%、100%乙醇浓度沉淀)制备灵芝胞外粗多糖,简称为 70%GLP、85%GLP、100%GLP,分别测定上述胞外多糖组分对白灵菇菌丝生长速度、高温胁迫后菌丝恢复速度、过氧化氢含量、丙二醛含量及胞内外酶活性的影响.结果表明,不同组分灵芝粗多糖中的总糖含量依次为 100%GLP＞70%GLP＞85%GLP.3.0 mg·mL-1 85%GLP对白灵菇菌丝生长的促进作用最明显,生长速度最快,可达 7.13 mm·d-1,且能显著提升白灵菇菌丝胞内外酶活性;添加灵芝胞外粗多糖可促进高温胁迫后白灵菇菌丝恢复,其中 85%GLP组分在长时间胁迫后恢复效果最优,12h恢复速度最快,达到 5.54 mm·d-1,同时能减少白灵菇菌丝中丙二醛和过氧化氢的积累,提升其抗逆性,从而减轻高温的负面效应.综上,灵芝胞外粗多糖可以促进白灵菇菌丝生长,且显著提升胞内外酶活性,同时缓解高温胁迫后菌丝内过氧化氢和丙二醛的积累.本研究结果为白灵菇产业高效稳定发展奠定了基础.
To shorten the growth cycle of Pleurotus tuoliensis while mitigating the adverse effects of high temperature on its mycelial growth.In this study,Ganoderma lucidum was used as the test material.Crude extracellular polysaccharides of G.lucidum(GLP)were prepared via liquid fermentation and precipitation with ethanol at concentrations of 70％,85％,and 100％,abbreviated as 70％GLP,85％GLP,and 100％GLP,respectively.The effects of these extracellular polysaccha-ride fractions on mycelial growth rate,recovery rate after high-temperature stress,hydrogen peroxide(H2O2)content,malo-ndialdehyde(MDA)content,and intracellular and extracellular enzyme activity of P.tuoliensis were determined.The results showed that the total sugar content among the different fractions of crude G.lucidum polysaccharides followed the order:100％GLP>70％GLP>85％GLP.The results showed that the total sugar content in the different crude GLP frac-tions was in the order of 100％GLP>70％GLP>85％GLP.The 85％GLP fraction at 3.0 mg·mL-1 exhibited the most significant promoting effect on the mycelial growth of P.tuoliensis,with the fastest growth rate reaching 7.13 mm·d-1,and significantly enhanced the intracellular and extracellular enzyme activity of the mycelia.The addition of crude GLP promoted the recovery of P.tuoliensis mycelia after high-temperature stress.Among them,the 85％GLP fraction showed the optimal recovery effect after prolonged stress,with the fastest recovery rate of 5.54 mm·d-1 at 12 h.Meanwhile,it reduced the accumulation of MDA and H2O2 in P.tuoliensis mycelia,improved its stress resistance,and thereby mitigated the negative effects of high temperature.In conclusion,crude extracellular polysaccharides from G.lucidum can promote the mycelial growth of P.tuoliensis,significantly enhance intracellular and extracellular enzyme activities,and alleviate the accumulation of H2O2 and MDA in mycelia under high-temperature stress.The results of this study lay a foundation for the efficient and stable development of the P.tuoliensis industry.
孙慧娴;张菁;高青云;郭昱秀;孙宁;班立桐;李奕葶
天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384天津农学院农学与资源环境学院 天津 300384
农业科技
灵芝胞外多糖白灵菇菌丝生长高温胁迫
Ganoderma lucidum exopolysaccharidePleurotus tuoliensisMycelial growthHigh-temperature stress
《中国瓜菜》 2026 (4)
127-133,7
天津市科技计划项目(23ZYCGSN00870,24ZYCGSN00070,24ZYCGSN00270)甘肃省2024年中央引导地方科技发展资金项目(24ZYQH006)甘肃省科技计划项目(24CXNH019)天津市蔬菜现代农业产业技术体系创新团队(ITTVRS2024016)
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