CRISPR/Cas9技术在茄科作物遗传改良中的应用研究进展OA

Gene editing technology provides a crucial tool for crop genetic improvement.As a third-generation gene edit-ing tool,the CRISPR/Cas9 system utilizes guide RNA to specifically recognize target sites and relies on the Cas9 nucle-ase to achieve precise editing:Such as gene knockout,replacement,or insertion—at the target gene locus.With advantag-es including simple design,strong targeting specificity,and high efficiency,the CRISPR/Cas9 gene editing tool has played a vital role in plant functional gene analysis and new variety creation.Solanaceae crops,such as potato,tomato,and pepper,are globally vital economic crops occupying a pivotal position in food security and agricultural development.However,their production is often constrained by factors including pests and diseases,abiotic stress,and quality defects.This paper outlines the fundamental principles of CRISPR/Cas9 technology,analyzes the challenges in editing efficiency and specificity,and reviews its application achievements in the genetic improvement of Solanaceae crops,aiming to pro-vide a scientific reference for precision breeding research and production practices in Solanaceae crops.