基于转录组数据解析红甜菜亚硒酸钠应答核心代谢通路OA

[Objective]To elucidate the regulatory mechanism of exogenous selenium on red beet,and determine the safe application concentration of sodium selenite,[Methods]this study established five sodium selenite treatments(1,3,6,10,and 15 mg/L,with water as the control).The effects on selenium uptake,transformation,and secondary metabolism in red beet were investigated by transcriptome sequencing coupled with GO/KEGG enrichment,and weighted gene co-expression network analysis(WGCNA).[Results]The results indicated that 3 mg/L sodium selenite was the optimal concentration for selenium biofortification in red beet,under which the selenium content in plants met the standard for selenium-enriched agricultural products.Treatments with different selenium concentrations induced a large number of differentially expressed genes,and higher selenium concentrations were more likely to activate selenium-responsive genes.GO and KEGG enrichment analyses revealed that selenium treatment significantly enriched pathways related to secondary metabolite biosynthesis.Notably,key genes in the phenylpropanoid biosynthesis pathway expressed differentially,potentially promoting the synthesis of stress-resistant compounds such as flavonoids.WGCNA identified multiple core co-expression modules associated with 3 mg/L selenium treatment,revealing that the Turquoise,Blue,and Green modules were enriched with secondary metabolism and stress response genes.[Conclusion]3 mg/L exogenous selenium is the optimal concentration for the safe and efficient selenium biofortification of red beet.Under this concentration,the selenium content in red beet meets the national standard for selenium-enriched crops,and it can activate genes related to secondary metabolism and stress response,thereby promoting the production of stress-resistant metabolites.This result provides theoretical support for the dosage regulation of sodium selenite in selenium-biofortified cultivation of red beet and research on selenium-mediated stress resistance mechanisms.