组蛋白去乙酰化酶抑制剂对甜菜抽薹开花相关基因表达的影响OA

[Objective]This study aimed to systematically explore the functional mechanism of histone deacetylases(HDACs)in the bolting and flowering process of sugar beets,and to analyze the regulatory effects of Zn2+-dependent HDACs inhibitors on the expression of flowering-related genes.[Methods]By applying HDACs inhibitors such as Tubastatin A and Quisinostat,the effects on the epigenetic modification status of beet flowering-related genes were analyzed.[Results]Semi-quantitative PCR results showed that Tubastatin A significantly promoted the expression of BvFT1,while significantly inhibiting the expression of BvBTC1,BvGI,and BvCO;Quisinostat had no significant effect on these genes;FNDR-20123 significantly promoted the expression of BvBTC1,BvGI,and BvCO;and Resminostat application led to a significant down-regulation of BvBTC1 expression levels.Quantitative real-time PCR results showed that Tubastatin A also significantly up-regulated the expression levels of BvBTC1 and BvGI,which was inconsistent with the semi-quantitative PCR results.HDACs inhibitors could significantly alter the histone acetylation levels of flowering genes by inhibiting enzymatic activity,thereby regulating the time course of sugar beet bolting and flowering.This study first established a direct association between the RPD3/HDA1 family and beet flowering regulation.[Conclusion]This study improves the epigenetic theoretical system of sugar beet flowering regulation,clarifies the regulatory network between HDACs activity and flowering gene expression,provides a technical path for early-maturing breeding and bolting-resistant variety improvement of sugar beets,and has paradigmatic reference value for the study of flowering mechanisms in long-day crops.