经皮穴位电刺激联合人绒毛膜促性腺激素治疗大鼠胚泡着床障碍的作用与机制OA

Objective To investigate the therapeutic effect and mechanism of transcutaneous electrical acupoint stimulation(TEAS)combined with human chorionic gonadotrophin(hCG)on blastocyst implantation dysfunction in rats.Methods Female rats were divided randomly into control,model,and treatment(TEAS+hCG)groups(n=6 per group).Rats in the treatment group received TEAS+hCG injections for 3 consecutive days starting from the late diestrus phase.On the day of proestrus,females were co-housed with male rats at a 2∶1 ratio.On gestation day 3,rats in the model and treatment groups received a subcutaneous injection of mifepristone suspension(5 mg/kg)to establish a blastocyst implantation dysfunction model.On gestation day 9,implanted blastocyst numbers,endometrial morphology,and expression levels of estrogen receptor α(ERα),progesterone receptor(PR),and leukemia inhibitory factor(LIF)were evaluated.Results The number of implanted blastocysts was significantly lower in the model group compared with the control group(P=0.005).The number was significantly increased after treatment with TEAS+hCG(P=0.027)compared with the model group.The endometrium was significantly thinner in the model group compared with the control group,with underdeveloped pinopodes.TEAS+hCG restored the endometrial thickness to a similar level to the control group,and improved the development of pinopodes.Expression levels of ERα mRNA(P=0.028),ERα protein(P=0.012),and PR protein(P=0.014)were significantly lower in the model group compared with the control group.Treatment with TEAS+hCG resulted in upward trends in the protein expression levels of ERα,PR,and LIF.Conclusions TEAS+hCG treatment may regulate endometrial expression of ERα,PR,and LIF,promote endometrial and pinopode development,improve endometrial receptivity,and effectively treat blastocyst implantation dysfunction in rats.