基于电化学传感与纳米材料的水产品中磺胺类药物残留快速检测技术研究OA

To address the urgent need for rapid on-site screening of sulfonamide residues in aquatic products,an electrochemical sensor was fabricated based on a screen-printed electrode(SPE)modified with a gold nanoparticle-reduced graphene oxide(Au-rGO)composite.After optimizing the modification process and detection conditions,differential pulse voltammetry(DPV)was used to evaluate the analytical performance of the sensor for sulfamerazine(SMZ)and sulfamethazine(SM2).During synthesis,ultrasonic time and centrifugal speed were controlled(12 000×g for 10 min)to effectively reduce background interference.SMZ exhibited linearity from 0.05 to 20.00 μmol/L(R2=0.997)with a limit of detection of 11.30 μg/kg.SM2 showed linearity from 0.08 to 25.00 μmol/L(R2=0.995)with a limit of detection of 15.60 μg/kg.The sensor demonstrated satisfactory sensitivity,selectivity,repeat-ability,and stability meeting the relevant analytical specifications.The anti-interference mechanism originated from the specific interactions and structural characteristics of the Au-rGO composite.These mechanisms were verified through repeated experiments.For actual sample detection,6 types of aquatic products,such as crucian carp and prawn were selected and analyzed for spiked recovery tests,with recoveries ranging from 93%to 105%.Comparison with high-performance liquid chromatography-mass spectrometry(HPLC-MS/MS)confirmed high consistency(R2=0.982).The total detection time for a single sample was approximately 35 minutes(30 minutes for pretreat-ment and 5 minutes for electrochemical detection).The consumable costs were approximately 20%of that traditional methods.Overall,these methods provide a practical technical solution for the rapid and accurate on-site determina-tion of sulfonamide residues in aquatic products.[Chinese Fishery Quality and Standards,2025,15(5):19-27]