初诊多发性骨髓瘤患者外周血T淋巴细胞中CD276的表达及临床意义OA

Objective:To investigate expression and clinical significance of negative costimulatory molecule CD276 on periph-eral blood CD4+T lymphocytes,CD8+T lymphocytes and Treg in newly diagnosed multiple myeloma(MM)patients.Methods:Periph-eral blood samples were collected from 34 MM patients hospitalized at the Second Hospital of Lanzhou University between September 2023 and October 2024 as experimental group,and from 16 healthy individuals as control group.Flow cytometry was used to measure CD276 expression on CD4+T cells,CD8+T cells and Treg,and correlations between CD276 expression and clinical characteristics or laboratory indices in MM patients were analyzed.Results:CD276 expression on CD4+T cells,CD8+T cells and Treg in peripheral blood of newly diagnosed MM patients were significantly higher than healthy control group(P<0.05).Further analysis showed that CD276 expression on CD4+T cells and Treg were associated with R-ISS stage of MM.Specifically,CD276 expression on CD4+T cells was significantly higher in patients with stage Ⅲ than in those with stage Ⅰ～Ⅱ(P<0.05),and CD276 expression on Treg was signifi-cantly higher in stage Ⅲ patients compared to those in stage Ⅰ～Ⅱ(P<0.05).Additionally,Treg CD276 expression was markedly higher in high-risk MM patients than standard-risk patients(P<0.05).CD276 expression on CD4+T cells and Treg were negatively cor-related with cytokine IL-4(r=-0.376,-0.402,P<0.05),while CD276 expression on CD8+T cells and Treg was negatively correlated with IL-8(r=-0.384,-0.365,P<0.05).Notably,CD276 expression on CD4+T cells,CD8+T cells and Treg was also significantly corre-lated with laboratory indices,including complement 3(C3),IgA,cholesterol(CHOL)and proportion of B cells.Conclusion:CD276 expression on CD4+T cells,CD8+T cells and Treg are significantly elevated in newly diagnosed MM patients and associates with clini-cal indicators such as R-ISS staging and disease risk stratification.Abnormal CD276 expression may contribute to pathogenesis of MM,with potential value as a prognostic marker and therapeutic target.