雷公藤多苷对过敏性紫癜肾炎大鼠的影响机制研究OA

Objective:To investigate the protective effect of tripterygium wilfordii glycosides(GTW)on kidney in rats with henoch-schonlein purpura nephritis(HSPN),and its regulation on polarization of M1/M2 macrophages mediated by Toll-like receptor 4(TLR4)signaling pathway.Methods:Rat model of HSPN was established by intragastric administration of bovine serum albumin(BSA)+LPS+carbon tetrachloride(CCl4),supplemented with dried ginger water.Experimental groups were as follows:control group,model group(HSPN),GTW low dose group(GTW-L),GTW high dose group(GTW-H),agonist group(LPS).Automatic biochemi-cal instrument was used to detect 24-hour urinary protein and renal function.ELISA was used to determine contents of IL-6 and TNF-α in serum of rats in each group.TUNEL staining was used to detect apoptosis rate in renal tissue,immunohistochemistry was used to de-tect the polarization of macrophages in rat renal tissue.Western blot was used to detect expressions of TLR4,myeloid differentiation factor 88(MyD88),NF-κB,p-NF-κB,Bcl-2 and Bax.Results:GTW could significantly reduce the quantity of 24-hour urinary pro-tein,improve renal function,decrease contents of IL-6 and TNF-α in serum,down-regulate rate of apoptosis in renal tissue,inhibit sig-nal transduction of TLR4,decrease expressions of TLR4,MyD88,NF-κB and Bax,increase expression of Bcl-2,while LPS could partially reverse the protective effect of GTW on renal tissue,and the differences were statistically significant(all P<0.05).Conclu-sion:GTW can significantly inhibit expressions of inflammatory mediators in renal tissue of HSPN rats,reduce apoptosis rate of renal tissue,reduce the pathological injury of renal tissue,and improve the renal function of rats,which may be related to its ability to inhibit the expression of TLR4 signaling pathway and promote the conversion of renal macrophages to type M2.