硫代牛磺酸抵御皮肤光损伤的作用机制研究OA

Objective To explore the repair mechanism of thiotaurine in resisting skin photodamage.Methods A model of light-induced damage was established using keratinocytes and a 3D epidermal model.The study assessed cell viability and Reactive Oxygen Species(ROS)levels post-light damage,as well as tissue vitality,immunofluorescence results for Filaggrin(FLG),Loricrin(LOR),and Lamin-B in the 3D epidermal model,and inflammatory factors.Results It can be known from the light-induced keratinocyte model that thiotaurine can enhance the cell viability after light damage and reduce the production of ROS.The light-induced 3D epidermal model repairs the epidermal barrier by increasing the expression of FLG and LOR,increases the expression of the nuclear senescence marker Lamin-B to resist cellular senescence,and can reduce the secretion of Interleukin-1 alpha(IL-1α)to reduce inflammation.Conclusion Thiotaurine can protect the skin from light-induced damage.