阿司匹林抑制瘢痕疙瘩成纤维细胞增殖并诱导细胞凋亡的作用研究OA

Objective As a benign fibroproliferative tumor,keloid is extremely prone to recurrence in the clinic regardless of surgical excision or drug injection,and there is no good treatment.Therefore,exploring and expanding more effective treatment modalities or drugs is an urgent clinical problem.Aspirin is a commonly used drug in the clinical treatment of cardiovascular and cerebrovascular diseases,and it has an antifibrotic effect,therefore,this paper initially explores whether it has an inhibitory effect on keloid scars,and whether it can be used as an alternative drug in the clinical treatment of keloid scars.Methods In this experiment,primary fibroblasts of keloid origin were obtained by the tissue block method,and the cells were collected for transcriptome sequencing after aspirin treatment,the effect of aspirin on the proliferation level of the cells was detected by the CCK-8 assay,and the cell cycle and apoptosis were detected by flow cytometry,and human tissue-derived xenograft models of keloid was constructed by using matrix gel to validate the tumorigenic ability of the cells after aspirin treatment.Results Transcriptome sequencing analysis showed that a total of 5 849 differential genes were detected in the aspirin-treated group compared with the control group,and the functions of the differential genes were mainly focused on the cell cycle,DNA replication,and the p53 signaling pathway,which all had a direct influence on the levels of cell proliferation and apoptosis;cell proliferation,cell cycle,and apoptosis assays demonstrated that aspirin inhibited the proliferation of fibroblasts of keloidal origin,induced apoptosis,and inhibited cell proliferation through cell cycle arrest;human tissue-derived xenograft models showed that the size of the xenografts became significantly smaller after aspirin treatment.Cell proliferation,cell cycle and apoptosis assays confirmed that aspirin inhibited the proliferation of keloid-derived fibroblasts and induced cell apoptosis,and inhibited cell proliferation through cell cycle arrest.Conclusion The present study demonstrates that aspirin can inhibit the proliferation and promote apoptosis of keloid-derived fibroblasts,and in vivo experiments in nude mice also demonstrates a significant reduction in the tumorigenic volume of the xenografts after treatment with aspirin,thus suggesting that aspirin is promising as a potential therapeutic agent for keloid scars.