响应面试验优化黑皮鸡枞液体菌种培养条件OA

As a newly emerging edible fungus successfully commercialised in recent years,Oudemansiella raphanipes is rich in a variety of nutrients,but the current research on the optimisation of the culture conditions of its liquid strains has not been thorough enough to achieve the optimal benefits.In this study,the optimal culture temperature、inoculum amount、shaker speed and incubation time were obtained by using one-way screening with the mycelial bi-omass of Oudemansiella raphanipes as the response value,and the optimal culture conditions were obtained and ver-ified by using Box-Behnken central composite test analysis.The results showed that the optimal culture conditions of Oudemansiella raphanipes were culture temperature 26℃,inoculum amount 11％,shaker speed 145 r/min and culture time 10 d.The biomass of mycelium could reach(1.213±0.022)g/100 mL,which was fitted with the theoret-ical value,indicating that the optimized culture conditions of Oudemansiella raphanipes liquid strain were reasona-ble.The study can lay the foundation for the efficient breeding and large-scale cultivation of the liquid strain.