质谱技术在临床药物体内定量分析中的应用与精准医疗实践进展OA

The process of clinical pharmaceutical quantitative analysis,represented by therapeutic drug monitoring(TDM),serves as a key component of precision medicine.Leveraging high sensitivity,high resolution,and multi-component analysis capabilities,mass spectrometry(MS)is gradually replacing traditional quantitative techniques or serving as an effective supplement to them in specific scenarios,significantly enhancing the accuracy and efficiency of TDM,and emerging as a core tool in clinical pharmaceutical quantitative analysis.In recent years,mass spectrometry technology has undergone rapid development.Beyond traditional triple quadrupole mass spectrometer(QQQ-MS),novel mass spectrometer techniques such as time-of-flight mass spectrometer(TOF-MS),orbitrap mass spectrometer(Orbitrap-MS),inductively coupled plasma mass spectrometer(ICP-MS),matrix-assisted laser desorption/ionization mass spectrometer(MALDI-MS),direct analysis in real-time mass spectrometer(DART-MS),and probe electrospray ionization mass spectrometer(pESI-MS)have emerged.These technologies integrate high throughput,high specificity,and real-time monitoring capabilities,and have become essential supporting technologies in precision medical practice.This review focuses on the principles and applications of the above six types of mass spectrometry technologies,aiming to advance TDM from an experience-driven approach to a data-driven one,thereby providing technical support for personalized medicine.