慢性肾脏病患者外周血调节性T细胞数量变化及其与血管钙化的相关性OA

Objective To explore the number of peripheral blood regulatory T cells(Treg)in patients with chronic kidney disease(CKD)and its correlation with vascular calcification.Methods This was a single-center,cross-sectional,and observational study.Non-dialysis patients with CKD treated at Zhongshan Hospital,Fudan University from March 2021 to March 2022 were enrolled.Abdominal aortic calcification(AAC)was assessed using lateral abdominal X-ray.Number of Treg and cytokine levels were measured by flow cytometry.Logistic regression analysis was performed to evaluate the related factors for AAC in CKD patients.Results A total of 83 patients were included,aged 17-86 years,with 57 males(68.7%).The distribution of CKD stages was as follows:stage G1 in 7 patients(8.4%),stage G2 in 17 patients(20.5%),stage G3 in 21 patients(25.3%),stage G4 in 19 patients(22.9%),and stage G5 in 19 patients(22.9%).No AAC was observed in patients with stages G1 and G2,while the prevalence of AAC in patients with stages G3,G4,and G5 was 23.8%,21.1%,and 26.3%,respectively.Compared with stage G1 patients,those with stages G3-5 showed decreased number of peripheral blood Treg and elevated levels of interleukin(IL)-6 and IL-17F(P＜0.05).The area under the receiver operating characteristic curve for number of peripheral blood Treg in predicting AAC in CKD patients was 0.766(95%CI 0.652-0.879,P=0.002).Logistic regression analysis showed that decreased number of Treg was related factor for AAC in CKD patients(OR=0.957,95%CI 0.922-0.992,P=0.018).Conclusion As CKD progresses,number of peripheral blood Treg significantly decreases,which is correlated with AAC in CKD patients.