髓母细胞瘤患儿脑脊液METTL3表达与预后的相关性研究OA

Objective To investigate the expression of methyltransferase-like 3(METTL3)in cerebrospinal fluids(CSF)and its correlation with the prognosis of children with medulloblastoma(MB).Methods Cerebrospinal fluids were collected from MB children.METTL3 protein levels were quantified using the Enzyme-Linked ImmunoSorbent Assay(ELISA)and its correlations with clinicopathological features were analyzed.The prognostic significance was evaluated via the Kaplan-Meier survival curve and Cox proportional hazards regression.Bioinformatics analysis was conducted to assess the association of METTL3 with chemotherapeutic drug sensitivities and functional enrichment of METTL3 in brain tumors.Results Ki-67 index was the only clinicopathological factor significantly correlated with CSF METTL3 levels(P＜0.05).METTL3 high expression group showed superior progression-free survival(PFS)(HR=0.376,95％CI:0.133-1.064,P＜0.05).Cox analysis identified METTL3 expression as an independent prognostic factor for PFS(P＜0.05).Significant negative correlations between METTL3 expression and 50％inhibitory concentrations(IC50)of methotrexate/vinblastine/etoposide(P＜0.001).Gene enrichment revealed METTL3's involvement in neural development and Notch signaling pathways.Conclusion METTL3 might serve as a prognostic biomarker for MB pediatric patients.Further validation with expanded cohorts and mechanistic studies(in vivo/in vitro)is warranted.