IgD对类风湿关节炎患者外周血诱导的单核-巨噬细胞极化、吞噬的影响OA

AIM:To investigate the effects of im-munoglobulin D(IgD)on the function of human pe-ripheral blood macrophages.METHODS:Peripheral blood mononuclear cells(PBMCs)were isolated from rheumatoid arthritis(RA)patients and healthy controls,and then adhered to the wall and stimulat-ed with macrophage colony-stimulating factor(M-CSF).Flow cytometry was used to detect the ex-pression of IgD Fc receptors(FcδR,IgDR)on the sur-face of peripheral blood mononuclear macrophages in RA patients and healthy controls.Stimulate macrophages with different concentrations of IgD(final concentrations of 1,3,10 μg/mL)for 48 hours,and flow cytometry was used to detect the expression of CD86 and CD206 on the surface of macrophages.Flow cytometry was used to detect the phagocytic of macrophages.RESULTS:Continu-ous expression of FcδR was detected on macrophages induced by peripheral blood mononu-clear cells,and the expression was higher in RA pa-tients than in healthy controls.The expression of M1 macrophages can be enhanced by IgD,while the expression of M2 macrophages can be weakened.At the same time,the phagocytic ability of macrophages are also enhanced with the increase of IgD levels.CONCLUSION:IgD may promote macrophage polarization in human peripheral blood monocytes by binding to FcδR,causing an imbal-ance in the proportion of M1 and M2 macrophages and enhancing phagocytic function of macrophages.