柴胡皂苷d逆转乳腺癌放疗抵抗的分子机制OA

Objective To investigate the molecular mechanisms of saikosaponin-d in reversing radioresistance of breast cancer.Methods The potential targets of saikosaponin-d were predicted using online databases,and differentially expressed genes associated with radioresistance of breast cancer were obtained from the GEO database.The intersecting targets were then screened,followed by network topology analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis.The online databases were employed to assess the expression differences of the core targets between normal breast tissue and breast cancer tissue,as well as their prognostic significance.Molecular docking technology was used to verify the interaction between saikosaponin-d and core targets.Results The potential targets of saikosaponin-d in reversing radioresistance of breast cancer mainly included TP53,HSP90AA1,MYC,HSP90AB1,EGFR,H3-3B,JUN,HDAC1,CDK2,TGFB1,mTOR,and RELA,and these targets were enriched in multiple pathways such as PD-1/PD-L1 and AGE-RAGE.The HPA database revealed that MYC,CDK2,and HSP90AB1 were highly expressed in breast cancer tissues,and the Kaplan-Meier Plotter database showed that H3-3B,TGFB1,mTOR and RELA were significantly related to the occurrence and prognosis of breast cancer.Molecular docking technology demonstrated that saikosaponin-d has good binding ability to the core targets.Conclusion Saikosaponin-d may reverse the radioresistance of breast cancer through multiple core targets,including H3-3B,mTOR,and RELA,as well as multiple pathways including PD-1/PD-L1 and AGE-RAGE,thereby providing a new theoretical basis for its clinical application.