一株丹顶鹤源鼠鼻罗氏菌的致病性及耐药性分析OA

In order to identity morbidity of red-crowned cranes in Yancheng National Rare Birds Nature Reserve in Jiangsu Province,and to develop reasonable countermeasures,oropharyngeal and cloacal swab samples were collected from affected cranes for pathogen isolation and identification,as well as pathogenicity and antibiotic resistance analysis.The results showed that a Gram-positive bacterium was isolated,which formed smooth,light gray,circular colonies on blood agar;positive reactions for glucose,citrate and amino acid metabolism were determined by biochemical identification tubes;bacterial DNA was extracted using a DNA extraction kit,and confirmed as Rothia nasimurium through 16S rDNA PCR amplification and sequencing.One-week-old ducklings were infected via oral for pathogenicity test,the symptoms of dose-dependent feather loss appeared one day post-infection,peaking after three days,and gradually recovering thereafter;characteristic pulmonary congestion and hemorrhage were revealed by post-mortem examination,but no significant lesions were observed in other organs.Disk diffusion antibiotic susceptibility test revealed that the strain was resistant to some aminoglycoside antibiotics,but sensitive to other classes of antibiotics.Pathogenic Rothia nasimurium was detected from a red-crowned crane population,and analyzed for its antibiotic resistance,providing a basis for pathogen monitoring of rare birds and disease prevention in the reserve.