不同磷水平下根瘤菌接种对饲用大豆生长和生理特性的影响OA

This study investigated the effects of rhizobial inoculation on the growth and physiological charac-teristics of forage soybean(Glycine soja Sieb.et Zucc'Hybrid F S006',hereafter referred to as S006)under dif-ferent phosphorus(P)levels.A pot experiment was conducted using vermiculite as the growth medium,with five P application rates:P0(0 g/kg),P1(0.36 g/kg),P2(0.54 g/kg),P3(0.71 g/kg),and P4(1.07 g/kg).At each P level,inoculated and non-inoculated(control,CK)treatments were established,and plant growth and physiological traits were measured 30 days after inoculation.The results showed that plant height,leaf length,leaf width,nodule number,nodule fresh weight,and catalase(CAT)activity,soluble sugar content all exhibited a unimodal response to increasing P supply,with peak values observed at the P2 level.This pattern indicated that low P supply promoted growth,whereas excessive P exerted an inhibitory effect.Rhizobial inocu-lation significantly improved plant growth and stress resistance across all P levels.Compared with the CK treat-ment,inoculation increased plant height by 50.44%-76.47%,enhanced CAT activity by 52.39%-111.50%,and reduced malondialdehyde(MDA)content by 16.38%-23.59%,thereby alleviating oxidative damage to cell membranes.In conclusion,the combination of rhizobial inoculation and the P2 level was most effective in pro-moting growth and improving physiological resistance in S006.These findings provide a theoretical basis for the coordinated application of phosphorus fertilizer and rhizobial inoculants in the efficient cultivation of forage soy-bean.