湖南稷子种质资源苗期耐盐性鉴定及耐盐种质筛选OA

This research evaluated 18 accessions of Echinochloa frumentacea(Roxb.)Link germplasm in a pot experiment under 150 mmol/L NaCl stress at the seedling stage to screen for salt tolerance and establish a corresponding evaluation system.Twenty-four phenotypic traits,encompassing biomass,anti-oxidant enzyme activity,chlorophyll fluorescence parameters and photosynthetic indices,were measured.The comprehensive salt tolerance index(D-value)of each accession was subsequently calculated.Principal component analysis(PCA),correlation analysis,membership function analysis,cluster analysis,and a comprehensive evaluation were then undertaken to systematically assess and classify the salt tolerance of the accessions.A predictive regression model for salt tolerance was developed using stepwise regression analysis.The results revealed significant differences between the salt-treated and control groups for all traits,with varying degrees of correlation among the 24 indicators.Based on the D-value-based hierarchi-cal cluster analysis,the 18 accessions were classified into four groups:two highly salt-tolerant accessions,ten salt-tolerant accessions,five moderately salt-tolerant accessions,and one salt-sensitive accession.Among the highly salt-tolerant group,accession CF033297 exhibited superior salt tolerance and was identified as an elite germplasm resources for breeding salt-tolerant Echinochloa frumentacea Stepwise regression analysis further iden-tified qP,K+,Pn,Fv/Fm,Proline content,O-2,aboveground biomass,and root length as reliable indicators for evaluating seedling-stage salt tolerance in Echinochloa frumentacea.