Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infectionsOA

作者：Peilan Wei;Bin Qu;Yuanyuan Zhang;Lei Chen;Zhiwei Xu;Zhao Chen;Qier Zhong;Xindan Xing;Xinxin Li;Jingjing Gao;Yifang He;Lu Zhang;Guifei Xie;Juan Shang;Xiaoke Guo;Jiaxin Jiang;Yongxia Shi;Jingxian Zhao;Yanqun Wang;Jincun Zhao;Yingkang Jin;Qingtao Hu;Airu Zhu;Zhen Zhuang;Zhaoyong Zhang;Shengnan Zhang;Jiantao Chen;Xinyi Xiong

作者单位：Pediatric Pulmonary Department,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou 510623,China||State Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,ChinaShanghai Institute for Advanced Immunochemical Studies,School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaHealth and Quarantine Laboratory,State Key Laboratory of Respiratory Disease of Guangzhou Customs District Technology Center,Guangzhou 510623,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaGMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,ChinaGMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaGuangzhou National Laboratory,Bio-Island,Guangzhou 510000,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||GMU-GIBH Joint School of Life Sciences,Guangzhou Medical University,Guangzhou 511436,China||School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 511436,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,China||Guangzhou National Laboratory,Bio-Island,Guangzhou 510000,China||Shanghai Institute for Advanced Immunochemical Studies,School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,ChinaPediatric Pulmonary Department,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangzhou 510623,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,China||China-Portugal Artificial Intelligence and Public Health Technologies Joint Laboratory,Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases,Guangdong Provincial Key Laboratory of Respiratory Disease Research,Guangzhou Medical University,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaState Key Laboratory of Respiratory Disease,National Clinical Research Center for Respiratory Disease,National Center for Respiratory Medicine,Joint International Research Laboratory of Respiratory Health,Guangdong Basic Research Center of Excellence for Respiratory Medicine,Guangzhou Institute of Respiratory Health,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510182,ChinaShanghai Institute for Advanced Immunochemical Studies,School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

中文关键词：Pediatric ARTIsMicrobial landscapesBronchoalveolar lavageMetatranscriptomicsAntimicrobial resistance

英文关键词：Pediatric ARTIsMicrobial landscapesBronchoalveolar lavageMetatranscriptomicsAntimicrobial resistance

刊名：《中国病毒学》 2026 (1)

页码/页数：58-69,12

基金：This project was supported by grants from the National Key Research and Development Program of China(2024YFC2310205 to L.Z.),the National Natural Science Foundation of China(82025001,82495200 and 82495203 to J.C.Z.,92369113 and 82172240 to Y.Q.W.),the Guangdong Medical Technology Research Foundation(B2022233 to Y.K.J.),the Guangzhou National Laboratory and State Key Laboratory of Respiratory Disease(GZNL2024B01001 to Y.Q.W.,and GZNL2023A01004 to Y.K.J.),the Guangdong Basic and Applied Research Projects(2023B1515020040 to Y.Q.W.),the State Key Laboratory of Respiratory Disease(SKLRD-Z-202411 to L.Z.,SKLRD-OP-202309 to Y.Q.W),the Science and Technology Planning Project of Guangzhou City(2024A03J1230 to J.C.Z.,2025B04J0006 to L.Z.),and the Science and Technology Project of General Administration of Customs,P.R.China(2025HK221,2023HK065 to L.Z.).We thank the Biobank for Respira-tory Disease in the National Clinical Research Center for Respiratory Disease(BRD-NCRCRD,Guangzhou,China).

DOI：10.1016/j.virs.2026.02.001