川楝子中1个新的柠檬苦素型三萜化合物OA

Objective To investigate the chemical constituents of limonoid-type triterpenes from the 95%ethanol extract of Melia toosendan and their antitumor activity.Methods The compounds were isolated and purified using various chromatographic techniques,including normal-pressure column chromatography,high-pressure column chromatography,and gel column chromatography.The isolated compounds were structurally characterized by modern spectroscopic techniques.Their antitumor activity was evaluated using the CCK-8 assay.Results Eight limonoid-type triterpenoids were isolated from the ethyl acetate extract of M.toosendan.Structural elucidation by spectroscopic techniques identified them as 15-oxotrichilinin(1),11,15-dioxotrichilinin(2),12-O-ethyl nimbolinin B(3),mufolinoid U(4),trichilinin(5),trichilinin D(6),trichilinin E(7),and trichilinin G(8).Activity evaluation results showed that all compounds exhibited varying degrees of inhibitory effects on four tumor cell lines.Conclusion Compound 1 is a new compound,named 15-oxotrichilinin.Antitumor activity results indicated that compounds 1-8 all possess antitumor activity to varying extents,with some compounds showing favorable activity against HepG2 cells.