响应面法优化大孔树脂纯化毛连菜中有机酸的工艺研究OA

Objective To optimize the purification process for organic acids from Picris hieracioides L.using macroporous resin by the Box-Behnken response surface methodology based on single-factor experiments.Methods Organic acids(pri-marily chlorogenic acid)extracted from P.hieracioides using deep eutectic solvents were selected.The optimal macroporous resin was screened by comparing the adsorption and desorption capacities of seven resins through static experiments.The effects of aspect ratio,eluent concentration,and elution volume on the purification were investigated using single-factor ex-periments.Subsequently,response surface methodology was employed to optimize these three critical parameters to deter-mine the optimal purification conditions.Results The NKA-2 macroporous resin was identified as the most suitable for pu-rifying organic acids.The optimal purification conditions were an aspect ratio of 8∶1,an eluent concentration of 80％,and an elution volume of 3 BV.Under these conditions,the total recovery rate of organic acids from P.hieracioides reached 90.38％,with a relative error of 0.64％compared to the predicted value.Conclusion The NKA-2 resin demonstrated ex-cellent performance in purifying organic acids from P.Hieracioides.The optimized process is stable and feasible,showing potential for industrial application.