硝普钠对哈密瓜采后品质及活性氧代谢影响的研究OA

To investigate the effects of sodium nitroprusside(SNP),a nitric oxide(NO)donor,on the quality and reactive oxygen species(ROS)metabolism of Hami melon during low-temperature storage[(3±1)℃],'Xizhoumi 25'Hami melons were used as test materials.Compared with the control,the fruits treated with 1.0 mmol·L-1 SNP maintained higher contents of soluble sugars,soluble proteins,titratable acids,and ascorbic acid during storage(28-42 days).This treatment effectively inhibited the accumulation of malondialdehyde(MDA)and the increase in cell membrane permeability,reduced the production rates of hydrogen peroxide(H2O2)and superoxide anion,and significantly enhanced the activities of antioxidant enzymes such as catalase(CAT)and superoxide dismutase(SOD).Additionally,it remarkably promoted the expression of genes including CmSOD,CmCAT,CmAPX,and CmPOD,thereby decreasing the cold damage index of Hami melon fruits and alleviating the degree of membrane lipid peroxidation.These results indicate that 1.0 mmol·L-1 SNP treatment can reduce cold damage and maintain fruit quality by regulating the balance of postharvest ROS metabolism in Hami melons.