毛花猕猴桃AeBAM基因家族鉴定及其表达分析OA

To elucidate the characteristics of the β-amylase(BAM)gene family in Actinidia eriantha,AeBAM family members were identified based on high-quality reference genome of A.eriantha'Ganlü 1'(PRJNA839193),and their physicochemical properties and evolutionary relationships were analyzed.In addition,transcriptome sequencing and qRT-PCR analyses were performed to determine the expression profiles of AeBAM genes in different tissues and at the late fruit development(starch degradation stage).A total of seventeen AeBAM genes were identified in A.eriantha,encoding proteins ranging from 189 to 751 amino acids,with theoretical isoelectric points of 5.24-10.01 and molecular weights of 21 149.85-84 142.08 Da.Phylogenetic analysis across many species classified BAM into five subgroups,with subgroups I and V containing the majority members.Collinearity analysis revealed 10 pairs of duplicated gene pairs and 5 pairs of tandemly duplicated genes pairs within the family.All members showed Ka/Ks ratios less than 1,indicating they were purifying selection during evolution.Promoter analysis indicated that AeBAM genes contained numerous cis-acting elements related to light responsiveness,stress adaptation,and hormonal regulation.Transcriptome analysis showed that AeBAM genes exhibited tissue-specific expression patterns,among which AeBAM5,AeBAM13 and AeBAM16 were highly expressed in fruits,and their expression levels significant increased after bagging.qRT-PCR validation revealed that the expression levels of AeBAM5 and AeBAM13 increased significantly during the starch degradation stage and were negatively correlated with starch degradation,suggesting that they may be key members of the AeBAM gene family regulating fruit starch degradation.