低共熔溶剂提取绞股蓝黄酮类成分的工艺优选OA

Objective To optimize the extraction process of rutin,quercetin,and kaempferol,three major flavonoid components,from Gynostemma pentaphyllum.Methods A HPLC method was used to analyze the content.The total extraction yield of three flavonoid components-rutin,quercetin,and kaempferol-was selected as the evaluation index.The optimal deep eutec-tic solvent system was determined by examining the type,molar ratio,and water content of the deep eutectic solvent.Box-Behnken response surface methodology was used to investigate the effects of liquid-solid ratio,ultrasonic temperature,and ultrasonic time on the extraction of Gynostemma pentaphyllum flavonoid components to determine the optimal extraction conditions.Results The optimum extraction process of three flavonoid components from Gynostemma pentaphyllum was as follows:the solvent composition consists of choline chloride and oxalic acid at a molar ratio of 3∶1,with a water content of 30%.Additionally,the liquid-to-solid ratio is maintained at 35 mL·g-1,the ultrasonic temperature is set at 69℃,and the ultrasonic duration is 40 minutes.Conclu-sion The optimized deep eutectic solvent-based extraction method demonstrates stability and feasibility,providing a reliable ap-proach for the efficient and safe extraction of flavonoids from Gynostemma pentaphyllum.