生物信息学视角揭示PYCR1基因在肿瘤免疫和预后中的作用OACHSSCD
Role of PYCR1 gene in tumor immunity and prognosis from the perspective of bioinformatics
目的 分析泛癌中吡咯啉-5-羧酸还原酶-1(PYCR1)基因的表达及其在肿瘤免疫、预后当中的作用,探讨 PYCR1 在肿瘤免疫治疗和临床预后中的价值.方法 利用 UALCAN、TCGA、GEO 和 GTEx 数据库对PYCR1 基因进行差异表达泛癌分析,通过 Kaplan-Meier 曲线及单因素 Cox 回归确定与预后的关系;CIBERSORT、ESTIMATE 评估与肿瘤免疫浸润的相关性;Spearman 分析 PYCR1 与肿瘤突变负荷、微卫星不稳定及免疫相关基因群的相关性;从 KEGG 数据库获得免疫相关过程基因群,进行相关性分析;GEO、HPA 数据库作为外部队列验证集.结果 PYCR1 基因在多种肿瘤中均处于高表达水平,生存分析显示其与多种肿瘤的预后密切相关,其中在ACC、KIRC、KIRP、MESO、PAAD、SARC、SKCM 中可以作为显著的危险因素,提示患者的不良预后;免疫浸润提示其在多种肿瘤中与免疫细胞浸润具有相关性,其中巨噬细胞和 T 细胞亚群与 PYCR1 表达量相关性最强;PYCR1 表达与 STES、STAD、LUAD、KIRC、KIPAN5 种肿瘤的突变负荷呈明显相关;PYCR1 表达与 UCEC、STES、STAD、KICA 的微卫星不稳定具有明显相关性;通路富集分析发现 PYCR1 参与了氨基酸代谢、氧化还原、细胞死亡等多个重要生物学过程;GEO、HPA 数据库验证了 PYCR1 在卵巢癌、宫颈癌、肝细胞癌中的差异表达.结论 PYCR1 有望作为泛癌肿瘤标志物,且可用于肿瘤免疫治疗效果评价以及评估肿瘤患者的预后,对临床肿瘤诊断、治疗及预后具有重要意义.
Objective To analyze the expression of pyrroline-5-carboxylate reductase 1(PYCR1)gene in pan-cancer and its role in tumor immunity and prognosis,and to explore the value of PYCR1 in tumor immunotherapy and clinical prognosis.Methods The UALCAN,TCGA,GEO,and GTEx databases were utilized for pan-cancer analysis of differential expression of PYCR1 gene.The relationship between PYCR1 gene expression and prognosis was determined through Kaplan-Meier curve and univariate Cox regression.CIBERSORT and ESTIMATE algorithms were used to evaluate the correlation with tumor immune infiltration.Spearman's rank correlation coefficient was used to analyze the association between PYCR1 and tumor mutational burden,microsatellite instability,as well as immune-related gene clusters.Immune-related process gene clusters were obtained from KEGG database for further correlation analysis.GEO and HPA databases were used as external cohort validation sets.Results PYCR1 gene was highly expressed in a wide variety of tumors,and survival analysis showed that it was closely related to the prognosis of a wide variety of tumors,among which ACC,KIRC,KIRP,MESO,PAAD,SARC,and SKCM could be used as a significant risk factor,indicating poor prognosis of patients.Immune infiltration suggested that PYCR1 was correlated with immune cell infiltration in a variety of tumors,and macrophages and T cell subsets were the most correlated with PYCR1 expression.PYCR1 expression was significantly correlated with the mutation load of STES,STAD,LUAD,KIRC,and KIPAN.There was a significant correlation between PYCR1 expression and microsatellite instability of UCEC,STES,STAD,and KICA.Pathway enrichment analysis showed that PYCR1 was involved in many important biological processes such as amino acid metabolism,REDOX,and cell death.GEO and HPA databases verified the differential expression of PYCR1 in ovarian cancer,cervical cancer,and hepatocellular carcinoma.Conclusion PYCR1 is expected to be a tumor marker of pan-cancer and can be used to evaluate the effect of tumor immunotherapy and evaluate the prognosis of cancer patients,which is of great significance for clinical diagnosis,treatment and prognosis of cancer.
任晨璐;马博乙;杨红
空军军医大学西京医院妇产科,陕西 西安 710032空军军医大学西京医院妇产科,陕西 西安 710032空军军医大学西京医院妇产科,陕西 西安 710032
医药卫生
PYCR1肿瘤免疫预后评估生物信息学泛癌
PYCR1tumor immunityprognosis evaluationbioinformaticspan-cancer
《空军军医大学学报》 2026 (4)
476-483,8
国家自然科学基金(82172993)陕西省自然科学基础研究项目(2024JC-YBMS-703)
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