益生元和益生菌干预对结直肠癌荷瘤小鼠化疗期间肠道菌群的影响OA

Objective To investigate the effect of prebiotic and probiotic on the structure of intestinal microbiota in colorectal cancer tumor-bearing mice during chemotherapy.Methods CT26 cells were subcutaneously injected into BALB/c mice to construct a tumor-bearing mice model of colorectal cancer.The mice were divided into control group(normal saline 0.5 ml/d),chemotherapy group(HS)group(20 mg/kg 5-FU),probiotic(HYSJ)group(bifidobacterium triple viable),prebiotic(HYSY)group(fructo-oligosaccharides enhance inulin),probiotic+prebiotic(HYSJYSY)group(bifidobacterium triple viable+fructo-oligosaccharides enhance inulin).After 3 weeks of continuous gavage of the above drugs,the animals were sacrificed and the tumor tissues were taken to evaluate the tumor volume and quality.The feces of each group of mice were taken for intestinal 16S rRNA V3 and V4 variable region gene sequencing.Results Compared with the control group,the tumor weight of mice in HS group,HYSJ group,HYSY group and HYSJYSY group was decreased,but the difference was not significant(P>0.05).Compared with HS group,the volume of HYSJ group,HYSY group and HYSJYSY group was decreased(P<0.05).HYSJYSY group had an effect on bacterial richness and uniformity,and had a significant effect on the composition of intestinal microflora.After 5-FU treatment,the microbial changes in the fecal samples of the model mice were analyzed.The results showed that compared with the HS group,the enrichment levels of Firmicutes,Bacteroidetes,Proteobacteriaand Fusobacteriain the HYSJYSY group were higher,while the enrichment levels of Tenericutes,Actinobacteria,TM7 and Verrucomicrobia were lower(P<0.05).Conclusion Prebiotic combined with probiotic can enhance the effect of chemotherapy,have anti-tumor effect and improve the intestinal microbial structure during 5-FU treatment period.