加味柴胡加龙骨牡蛎汤治疗卒中后抑郁模型大鼠疗效及对突触可塑性的影响OA

Objective:To observe the therapeutic effect of Modified Chaihu Plus Longgu Muli Decoction on PSD model rats and its impact on synaptic plasticity,and to explore the mechanism of this formula in treating PSD.Methods:Fifty male SD rats were randomly allocated to five groups(n=10 each):sham-operated group,middle cerebral artery occlu-sion(MCAO)group,PSD group,positive control group(fluoxetine hydrochloride 1.58 mg·kg-1),and traditional Chi-nese medicine group(Modified Chaihu Plus Longgu Muli Decoction 11.7 g·kg-1).Cerebral infarction models were es-tablished using middle cerebral artery occlusion,and PSD models were successfully prepared by applying chronic un-predictable mild stress(CUMS)and solitary housing based on this model.After successful modeling,drugs were ad-ministered by gavage for 3 consecutive weeks.Depressive-like behaviors were assessed using the open field test,neuro-nal counts in the hippocampal CA1 region were detected by Nissl staining,PSD95 and SYN protein expression in the hippocampal CA1 region was examined via immunohistochemistry,and BDNF and VEGFA protein levels in hippocam-pal tissues were measured by Western blot.Results:Open field test results showed that central zone duration and movement distance in the PSD group were significantly lower than those in the sham-operated and MCAO groups(P<0.05),while both the traditional Chinese medicine(TCM)and positive control groups exhibited significantly higher values than the PSD group(The duration of center zone activity in the Chinese materia medica group was prolonged by 378%(MD=17.23 s,95%CI:12.4-22.1)compared with the PSD group;the movement distance was increased by 261%(MD=2.09 m,95%CI:1.74-2.44))(P<0.05).Nissl staining revealed significantly fewer neurons in the MCAO and PSD groups compared to the sham-operated group(P<0.05),with higher neuronal counts in the positive control and Chinese materia medica groups versus the MCAO and PSD groups(P<0.05).Immunohistochemistry in-dicated lower SYN and PSD95 expression in the MCAO and PSD groups than in the sham-operated group(P<0.05).The positive control group showed higher SYN expression than the MCAO and PSD groups(P<0.05),while the Chi-nese materia medica group demonstrated elevated expression of both SYN and PSD95 compared to the MCAO and PSD groups(P<0.05).Western blot analysis showed reduced BDNF and VEGFA protein expression in the MCAO and PSD groups relative to the sham-operated group(P<0.05),whereas the positive control and Chinese materia medica groups exhibited increased expression of these proteins compared to the disease model groups(P<0.05).Conclusion:Modified Chaihu Plus Longgu Muli Decoction improves synaptic microenvironment and enhances synaptic plasticity,thereby reducing hippocampal neuronal loss and ameliorating depressive behaviors in PSD rats.