基于网络药理学探究中风康复膏治疗血管性认知障碍的作用机制OA

Objective:To explore the mechanism of vascular cognitive disorders treated by stroke rehabilitation ointment using network pharmacology,and to provide theoretical basis for the use of the formula.Methods:Firstly,the TCMSP database was used to screen the active ingredients and corresponding targets of the stroke rehabilitation ointment,and the GeneCards,DisGeNET and OMIM databases were used to search for"vascular cognitive disorders"and obtain the information of disease-related targets.The intersection of drug and disease targets was taken and a Wayne diagram was made,and a protein-protein interaction(PPI)network diagram was constructed using Cytoscape software to screen the key components and core genes,and a protein-protein interaction network was constructed in the STRING database,and then clustering analysis was carried out using the MCODE plug-in.The core targets were then analyzed for Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment using Metas-cape database.Results:The stroke rehabilitation ointment was screened to contain 99 active ingredients,corresponding to 403 targets.There were 1 165 targets related to vascular cognitive disorders,of which 165 drugs intersected with dis-ease targets.GO functional enrichment analysis identified 5 690,493 and 891 genes related to biological processes,cel-lular components and molecular functions,respectively.In KEGG analysis,cancer pathway,AGE-RAGE signaling path-way,and MAPK signaling pathway were the most common pathways.Conclusion:Quercetin,stigmasterol,lignans,rhodopsin methyl ether,and β-sitosterol in stroke rehabilitation ointment can regulate MAPK,AGE-RAGE,and cancer signaling pathways through multiple targets such as IL-6,AKT1,TNF,IL-1β,and TP53 for the treatment of vascular cognitive impairment.