管花肉苁蓉配方茶的提取工艺及其抗疲劳作用研究OA

Objective:To investigate the optimal extraction protocol of Cistanche tubulosa(Schenk)Wight Formula Tea and assess its functional efficacy in mitigating physical fatigue in mice,thereby laying a foundation for subsequent in-depth research on the anti-fatigue mechanisms of Cistanche tubulosa(Schenk)Wight Formula Tea.Methods:An or-thogonal experimental design was employed,with the yield of total saponins as the key indicator to ascertain the opti-mal extraction parameters.Through murine anti-fatigue functional assays,the impacts of Cistanche tubulosa(Schenk)Wight Formula Tea on mice's loaded swimming duration,serum urea content,hepatic glycogen storage,and blood lactic acid levels were examined to evaluate its anti-fatigue efficacy.Results:The optimal extraction conditions for Cistanche tubulosa(Schenk)Wight Formula Tea were identified as 15 times the volume of water,with each extraction lasting 1 hour and repeated 3 times.Test results regarding sensory attributes,physicochemical indices,and microbial indicators of Cistanche tubulosa(Schenk)Wight Formula Tea all conformed to national standards.The signature components,namely tea polyphenols and total saponins,were determined to be 7.5g and 0.6g per 100g,respectively.Findings from murine functional experiments revealed that at a dosage of 4.50 g/kg,Cistanche tubulosa(Schenk)Wight Formula Tea could markedly extend the loaded swimming time of mice,enhance hepatic glycogen reserves,and lower post-exercise serum urea concentrations(P<0.05).No statistically significant influence of each dosage on the area under the blood lactic acid curve in mice was detected(P>0.05).Conclusion:The extraction protocol of Cistanche tubulosa(Schenk)Wight Formula Tea exhibits favorable stability and reproducibility.Under this extraction protocol,Cistanche tubulosa(Schenk)Wight Formula Tea demonstrates a significant efficacy in alleviating physical fatigue in mice.The findings of this study offer reliable support for process parameters,facilitating its functional development and practical application.