17个春兰品种叶片叶绿素含量和解剖结构比较OA

The chlorophyll content and anatomical structure of leaves of different varieties of Cymbidium goeringii were compared to provide basic data and theoretical reference for their cultivation management,variety improvement and leaf function analysis.Seventeen varieties of C.goeringii were selected to determine the chlorophyll content,stomatal structure and cross-sectional structure of their leaves by using the SPAD chlorophyll meter method,nail polish method and freehand sectioning method.The results showed that there were significant differences in chlorophyll content among the varieties of C.goeringii,with SPAD values ranging from 43.07 to 67.84.Among them,the SPAD value of'Jinxiu Zhonghua'was the lowest and that of'Lvying'was the highest.The stomatal structure of leaves of C.goeringii showed that the stomatal long axis of'Jinxiu Zhonghua'was the smallest and that of'Xueshan'was the largest;the stomatal short axis and stomatal area of'Datuanyuan'were the smallest and those of'Xiaodamei'were the largest;the stomatal density of'Xueshan'was the smallest and that of'Huanqiu'was the largest.The cross-sectional structure of leaves of C.goeringii showed that the upper epidermis thickness of'Datuanyuan'was the smallest and that of'Longzi'was the largest;the lower epidermis thickness of'Jinxiu Zhonghua'was the smallest and that of'Zhonghua Yipinmei'was the largest;the long axis of leaf vein of'Tianpeng Mudan'was the smallest,the short axis of leaf vein of'Xiaodamei'was the smallest,and the long axis and short axis of leaf vein of'Dayuanbao'were the largest;the leaf thickness of'Jinxiu Zhonghua'was the smallest,the mesophyll thickness of'Yuhuadie'was the smallest,and the leaf thickness and mesophyll thickness of'Guiyuan'were the largest.The correlation analysis revealed varying degrees of correlation among the leaf indicators of C.goeringii.The SPAD value showed a highly significant positive correlation with leaf thickness,and significant positive correlations with short axis of leaf vein and mesophyll thickness.The stomatal area demonstrated a highly significant positive correlation with stomatal long axis and stomatal short axis,while showing a significant negative correlation with short axis of leaf vein.The long axis of leaf vein exhibited a highly significant positive correlation with short axis of leaf vein.The leaf thickness demonstrated a highly significant positive correlation with mesophyll thickness.There were significant differences in chlorophyll content and anatomical structure among seventeen varieties of C.goeringii,indicating that there might be significant differences in photosynthetic capacity and stress resistance among the varieties.