海水胁迫下黄秋葵种子发芽率的全基因组关联分析OA

To explore the candidate genes related to salt tolerance in okra under seawater stress,180 okra(Abelmoschus esculentus)germplasm resources from various sources were used as materials,and the seeds were treated with seawater at 3‰ and 6‰ salinity.The germination percentage and relative germination percentage were measured,genome-wide association study(GWAS)was performed with SNP markers,and candidate gene screening was performed by transcriptome sequencing(RNA-seq).The results showed that 3‰ and 6‰ salinity of seawater had different degrees of inhibition on the germination rate of okra seeds.A total of 75 SNP loci were screened out by GWAS.An association interval was detected on chromosome 54,and four SNP loci were detected simultaneously under seawater stress of 3‰ and 6‰ salinity,which were important loci for controlling the relative germination rate and salt tolerance,and a total of 54 annotated genes were detected within 100 kb of the upstream and downstream of the four SNPs.Based on the results of transcriptome sequencing under seawater stress at the seedling stage of okra,six differentially expressed genes were screened out as candidate genes for salt tolerance of okra under seawater stress,namely Ae54G020550,Ae54G020570,Ae54G020630,Ae54G020730,Ae54G020810 and Ae54G020890,and the annotation functions were cytoskeletal composition,encoding CYSTM protein,NPH3 or BTB/POZ protein,alcohol dehydrogenase,leucine-rich repeats,and PPR family proteins.These results provide a reference for analyzing the function of salt tolerance genes in okra and the molecular mechanism of salt tolerance.