光敏剂BC诱导细胞凋亡抑制结直肠癌机制研究OA

Objective To investigate the effect and mechanism of photosensitizer BC on the proliferation,migration and invasion of colorectal cancer cells.Methods Firstly,the inhibitory effect of photosensitizer BC on the proliferation of colorectal cancer cells under both dark and light conditions was evaluated by an MTT assay,and its half inhibitory concentration IC50 was determined.The cells were grouped accordingly for subsequent experiments.Scratch wound healing and trans well invasion/migration assays were utilized to demonstrate the in-hibitory effects of BC on the invasive and migratory capabilities of colorectal cancer cells;intracellular reactive oxygen species(ROS)levels were measured to assess oxidative stress;changes in mitochondrial membrane potential were detected;and Western blot analysis was per-formed to examine the expression levels of Bcl-2 and Bax proteins in the colorectal cancer cells.Results Compared to dark conditions,BC significantly inhibited the proliferation,migration and invasion of colorectal cancer cells under light conditions.It induced the increase of ROS level and the decrease of mitochondrial membrane potential.Furthermore,it modulated the protein expression levels,downregulating Bcl-2 while upregulating Bax.Conclusion The activity of photodynamic compound BC after light activation is significantly better than that under dark conditions,and it can inhibit the proliferation,invasion and migration of colorectal cancer.It may induce apoptosis of colorectal cancer by generating increased levels of ROS,thereby affecting mitochondrial membrane potential.