患病绵羊肺组织关键病原微生物宏基因组解析与谱系特征研究OA

This study aimed to characterize pathogenic microorganisms causing infectious respiratory diseases in sheep,to investigate the relationship between sheep breeds and microbial homologous genomic expression,and to lay a foundation for disease-resistant breeding and efficient production in sheep.Thirty-six 180-day-old Australian Dorper-Lambeth crossbred sheep and 25 Molang sheep,reared under iden-tical conditions,were selected.A total of 61 lung tissue samples were collected from the sheep.Then,metagenomic techniques were em-ployed to analyze the microbial community composition within the lung tissues.Next,PCR amplification techniques were used to identify ma-jor pathogenic microorganisms.Concurrently,pro-inflammatory cytokine expression was measured to analyze differences in microbial homolo-gous genome expression between the sheep breeds.The results showed that thirteen key respiratory system microorganisms were identified in the sheep lung tissue.The PCR amplification confirmed sheep lung adenovirus as the primary pathogenic microorganism in this population.The homologous genome expression analysis indicated that the microbial homologous genome energy in Australian Dorper sheep was primarily allocated to RNA processing and modification,whereas in Duolang sheep,it was mainly directed towards carbohydrate metabolism,transport and metabolism of secondary metabolites,and defense mechanisms.The pro-inflammatory cytokine detection revealed significant differences in gene expression and energy allocation between the two sheep breeds.The correlation analysis exhibited a high degree of association between sheep breed and functional expression of lung tissue microbiota.In conclusion,sheep lung adenovirus constituted the primary pathogenic microorganism responsible for infectious respiratory diseases within this sheep population.Microbial homologous genome expression exhibited breed specificity between Australian Dorper and Duolang sheep,with sheep breed being closely associated with functional expression of lung tissue microbiota.