新西兰兔发情与结肠微生物关联性研究OA

The aim of this study was to investigate the relationship between estrogen related to estrus and gut microbiota in New Zealand white rabbits.Female rabbits in diestrus(UN)and estrus(ES)were selected by visual estrus detection based on vulval changes,plasma es-tradiol was measured by enzyme-linked immunosorbent assay(ELISA),and metagenomic detection methods were used to determine the composition and function of colonic microorganisms and estrus status,and the correlation between them was analyzed.The result were as fol-lows:After conducting species annotation and functional analysis on the samples through metagenomic sequencing,it was found that the abundance of the colonic microbiota in New Zealand white rabbits UN was significantly higher than that in ES(P＜0.05).Among them,Al-phaproteobacteria was the representative species of the ES group,Pseudoflavonifractor and Acetatifactor muris were the representative species of the UN group.The KEGG functional difference analysis showed that the abundance of microorganisms related to amino acid metabolism and carbohydrate metabolism was higher in the UN group,while the relative abundance of glycan biosynthesis and metabolism,cell growth and death,and signal transduction was higher in the ES group.The difference analysis of CAZy showed that glycoside hydrolases were significant-ly enriched in the UN group(P＜0.05),while polysaccharide lyase,glycoesterase,and oxidoreductases were significantly enriched in the ES group(P＜0.05).The correlation analysis showed that colonic microbial composition and function were significantly correlated with estrous status and plasma E2 levels(P＜0.05).In summary,this study preliminarily demonstrated that there were significant differences in colonic microbial composition in different estrus states,which might serve as a direction for further research on the mechanism of estrus in New Zeal-and white rabbits.