畜禽养殖粪便中31种多类抗生素残留的测定OA
Determination of 31 Multi-class Antibiotic Residues in livestock and Poultry Breeding Manure
通过比较,优化前处理方法,建立一种固相萃取—高效液相色谱串联质谱快速检测畜禽粪便(鸡粪)中磺胺类、四环素类、大环内酯类、喹诺酮类、氯霉素类共5大类31种抗生素的检测方法.样品经提取液萃取后,通过HLB小柱对样品进行富集和净化.液相色谱选用C18柱,以0.2%甲酸—水和乙腈作为流动相对样品进行梯度洗脱分离.该方法31种抗生素的检出回收率为58.9%～97.7%,相对标准偏差为3.2%～11.5%.该方法适用于畜禽粪便(鸡粪)中多类抗生素的残留检测.
Through the comparison and optimization of the pretreatment methods,a rapid detection method for 31 antibiotics,including sulfonamides,tetracyclines,macrolides,fluoroquinolones and chloramphenicols,in livestock and poultry manure(chicken manure)was established via solid-phase extraction(SPE)combined with high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were enriched and purified by an HLB(Hydrophilic-Lipophilic Balance)column after sample extraction.The separation was performed on a C18 column with gradient elution,using 0.2%formic acid aqueous solution and acetonitrile as the mobile phases.The recoveries of the 31 antibiotics ranged from 58.9%to 97.7%,and the relative standard deviations(RSDs)were 3.2%to 11.5%.This method is applicable to the determination of multi-class antibiotic residues in livestock and poultry manure(chicken manure).
张琳;胡潇涵;沈丽丽
新疆维吾尔自治区生态环境科学研究院,新疆 乌鲁木齐 830011||新疆环境污染监控与风险预警重点实验室,新疆 乌鲁木齐 830011||新疆清洁生产工程技术研究中心,新疆 乌鲁木齐 830011||新疆生态环境科学研究院司法鉴定中心,新疆 乌鲁木齐 830011新疆维吾尔自治区生态环境科学研究院,新疆 乌鲁木齐 830011||新疆环境污染监控与风险预警重点实验室,新疆 乌鲁木齐 830011||新疆清洁生产工程技术研究中心,新疆 乌鲁木齐 830011||新疆生态环境科学研究院司法鉴定中心,新疆 乌鲁木齐 830011新疆维吾尔自治区生态环境监测总站,新疆 乌鲁木齐 830000
资源环境
高效液相色谱—串联质谱法固相萃取抗生素畜禽粪便
HPLC-MS/MSsolid-phase extractionantibioticslivestock and poultry manure
《新疆环境保护》 2026 (1)
64-72,9
新疆维吾尔自治区自然科学基金面上项目(2022D01A114)
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