青霉素降解菌的筛选鉴定及其降解特性研究OA

Five penicillin-resistant strains were isolated and screened from the soil applied with penicillin residue organic fertilizer from a pharmaceutical factory,and their penicillin degradation efficiency was determined.One penicillin-efficient degrading bacterium was selected to study its morphology,physiological and biochemical characteristics and biosafety.The results showed that strain 127-1(Bacillus wiedmannii,NR_152692.1),a highly efficient penicillin-degrading bacterium,was identified as Bacillus wiedmannii by 16S rDNA sequence molecular identification.The degradation rate of penicillin with high concentration(1000 μg/mL)by strain 127-1 was as high as 99.93%within 48 hours.Strain 127-1could grow at 4～45℃,and the optimum growth conditions were pH=8.0 and salinity<3%.In addition,strain 127-1 has good growth-promoting functional characteristics and strong environmental adaptability,showing excellent application potential.