陕西省临潼区石榴病毒病的发生与分布OA

Pomegranate(Punica granatum L.)is an important economic crop,and its production and industry development are severely constrained.To identify the causal viruses,their distribution,and associated symptom characteristics of pomegranate viral diseases in the main production areas of Lintong District,Shaanxi Province,leaf samples showing suspected viral infection were collected from pomegranate orchards.Deep sequencing analysis combined with RT-PCR detection was conducted.The results showed that three viruses were associated with pomegranate viral diseases in the Lintong pro-duction area,including Apple latent spherical virus(ALSV),Passiflora edulis symptomless virus(PESV),and Humulus japonicus latent virus(HJLV).PESV infection was associated with chlorosis,as well as leaf reduction and curling,whereas ALSV infection was associated with leaf malformation.Viruses were detected in some cutting-propagated seedlings and grafted seedlings,indicating that or-chard establishment using infected seedlings or grafting with virus-infected scions is the main route of virus transmission in pomegranate orchards.The detection rates of ALSV,HJLV,and PESV ranged from 33.3％-46.7％,30.0％-36.7％,and 16.7％-30.0％,respectively,and mixed infections invol-ving the three viruses were common.This study provides the first evidence of pomegranate viral disea-ses in China and clarifies the causal virus species,regional distribution,and symptom characteristics of pomegranate viral diseases in Lintong,Shaanxi,thereby providing important theoretical evidence and technical support for accurate diagnosis and targeted control of pomegranate viral diseases in China.