不同生境当归茎叶内可培养微生物群落组成和多样性研究OA
Composition and Diversity of Culturable Microbial Communities in Stems and Leaves of Angelica sinensis from Different Habitats
采用传统分离培养技术,对甘肃省漳县(ZX)、岷县(MX)和宕昌(TC)3个主产区当归茎部和叶部的内生菌多样性及群落结构进行分析.结果显示,从当归茎部和叶部共分离得到435株内生细菌,隶属于35属108种;297株内生真菌,隶属于15属89种.TC样地叶部的内生细菌与内生真菌丰富度和多样性均为最高,而ZX样地茎部细菌群落和叶部真菌群落多样性最低.各生境当归内生细菌优势属有芽孢杆菌属、假单胞菌属和赖氨酸芽孢杆菌属,其中,芽孢杆菌属在ZX样地叶部富集,假单胞菌属在TC样地叶部占优.内生真菌优势菌属为镰刀菌属、交链孢霉属和类茎点霉属,镰刀菌属在3个生境的茎部普遍富集,而交链孢霉属在ZX和MX样地叶部占优势.Pearson相关性分析表明,不同生境当归茎部细菌群落结构在属水平上相关性较小,真菌则存在极显著相关性(P＜0.001).此外,MX和TC样地叶部内生细菌群落存在显著正相关性(P＜0.05),ZX和MX叶部内生真菌群落之间存在极显著正相关性(P＜0.001).主成分分析和对应分析显示,生境差异和组织部位特异性共同影响内生菌的群落结构.
In this study,traditional isolation and culture-based methods were used to characterize the diversity and community structure of endophytic microorganisms(bacteria and fungi)in the stems and leaves of Angelica sinensis from three major production regions in Gansu province,China:Zhangxian(ZX),Minxian(MX),and Tanchang(TC)counties.The results showed that a total of 435 endophytic bacterial strains,belonging to 35 genera and 108 species,and 297 endophytic fungal strains,belonging to 15 genera and 89 species,were isolated from the stems and leaves of A.sinensis.The richness and diversity of both endophytic bacteria and fungi were the highest in the leaves from the TC region,whereas the diversity of the bacterial community in the stems and the fungal community in the leaves from the ZX region was the lowest.The dominant endophytic bacterial genera in A.sinensis from vari-ous habitats were Bacillus,Pseudomonas and Lysinibacillus.Bacillus was enriched in the leaves of the ZX region,while Pseudomonas was dominant in the TC leaves.The dominant fungal genera were Fusarium,Alternaria,and Calophoma.Fusarium was consistently enriched in the stems across all three habitats,while Alternaria was predominated in the leaves of the ZX and MX regions.Pearson correlation analysis indicated that the bacterial community structures in the stems exhibited relatively weak correlations at the genus level among different habitats,whereas the fungal communities showed a highly significant correlation(P＜0.001).In addition,a significant positive correlation(P＜0.05)was observed in the endophytic bacterial communities of leaves from the MX and TC plots,while an extremely significant positive correlation(P＜0.001)was found in the endophytic fungal communi-ties of the leaves from the ZX and MX plots.Principal component analysis(PCA)and correspondence analysis(CA)indicated that both habitat differences and tissue-specificity jointly influenced the struc-ture of endophytic microbial communities in the stems and leaves of A.sinensis.
晏祖花;金辉;杨晓燕;华翠平;许忠祥;贺晓倩;彭雪枫;秦波
中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000||中国科学院大学,北京 100049中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000南京海关动植物与食品检测中心,南京 210026中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000||中国科学院大学,北京 100049中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000||中国科学院大学,北京 100049中国科学院兰州化学物理研究所,天然药物与化学测量研究中心,兰州 730000
当归内生菌多样性群落组成生境
Angelica sinensisEndophytesMicrobial diversityCommunity structureHabitat
《西北农业学报》 2026 (4)
724-735,12
甘肃省知识产权计划项目(24ZSCQG013)甘肃省科技计划项目(23JRRA604,24JRRA065). Gansu Provincial Intellectual Property Plan(No.24ZSCQG013)Gansu Provincial Science and Technology Program(No.23JRRA604,No.24JRRA065).
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