野生西藏沙棘多功能菌株的筛选鉴定及促生能力比较OA

In this study,rhizosphere soil samples of Hippophae thibetana Schlechtend(Wild Tibetan Seabuckthorn)from Qinghai Province were collected to isolate,purify,and classify microbial strains.The functional abilities of the strains,including nitrogen fixation,phosphorus solubilization and po-tassium release,were evaluated.The growth-promoting effects of selected multifunctional strains were also assessed.The results showed that three strains of Pseudomonas(N7,W4,N19)and one strain of Enterohepater(Y65)were identified.After five days of cultivation,the diameter of the nitro-gen-fixing halo ranged from 9.07 to 11.63 mm,and nitrogenase content ranged from 183.41 to 241.38 U/L.The diameter of the phosphorus-solubilizing halo ranged from 6.80 to 16.43 mm,with available phosphorus content ranging from 13.17 to 18.77 μg/mL.The diameter of the potassium-re-leasing zone ranged from 14.43 to 19.41 mm,and available potassium content ranged from 18.47 to 21.47 μg/mL.The results of the growth-promoting test showed that all four strains increased the ger-mination rate of lettuce,with the germination rates ranging from 78.89％to 90.00％.All strains ex-hibited significant abilities of nitrogen fixation,phosphorus solubilization,potassium release,and growth promotion.Among them,strain Y65 showed the most significant effect on biomass accumula-tion.