高温胁迫对小麦花药细胞程序化死亡的影响及TaMCA4基因表达分析OA

High-temperature is a major environmental stress factor affecting wheat growth and devel-opment.To investigate the regulatory mechanisms of programmed cell death(PCD)occurring during high-temperature induced anther abortion,anthers at different developmental stages(late-uninucleate stage,binucleate stage,and trinucleate stage)were collected from wheat grown under control and high-temperature stress conditions.Morphological changes in the tapetum and DNA fragmentation were comparatively analyzed between treatments.In addition,the expression patterns of the PCD-re-lated gene TaMCA4 were also investigated.The results showed that under high-temperature stress,the cytoplasmic density of the tapetum began to diminish from the late-uninucleate stage.By the tri-nucleate stage,the tapetum had completely degraded,indicating the premature degradation of the ta-petal cells.This process was accompanied by a characteristic DNA laddering pattern,a hallmark of PCD.During anther development,the expression of TaMCA4 was suppressed at the late uninucleate stage under high-temperature stress,resulting in relatively low expression levels.However,TaM-CA4 expression was significantly upregulated at binucleate and trinucleate stages,suggesting its in-volvement in the PCD process during high-temperature stress induced anther abortion.Protein-protein interaction analysis predicted ten proteins interacting with TaMCA4,among which the glycosyltrans-ferase family protein A0A0R6UPC9 exhibited the highest interaction score.These results may provide new insights into the molecular mechanisms of high-temperature induced anther abortion in wheat.