盐胁迫下辣椒幼苗根系的转录组分析OACHSSCD

To explore the molecular mechanism underlying the response of Capsicum annuum L.seedlings to salt stress,this study used the pepper cultivar'Dingcui'as experimental materials and subjected the seedlings to 150 mmol∕L NaCl treatment for 24 h.Transcriptome sequencing was subsequently conducted to analyze the changes in root gene expression profiles.The results showed that salt stress induced 2 214 differentially expressed genes,which were significantly enriched in multiple biological pathways including phenylpropanoid biosynthesis,response to water deprivation,and cutin∕wax biosynthesis.Further analysis of transcription factors re-vealed that the MYB,bHLH and ERF families were markedly activated,with MYB family members(e.g.,Capana02g003250,log2 Fold Change=7.28)occupying a core position in the regulatory network.Collectively,these findings indicate that C.annuum seedlings primarily adapt to salt stress through enhanced secondary metabolism and physical barrier construction driven by a MYB-cen-tered transcriptional regulatory network,and their salt tolerance mechanism exhibits species-specific characteristics.This research pro-vides key candidate genes and a theoretical basis for the genetic improvement of salt tolerance in pepper crops.