日光温室草莓与番茄高效间作模式对基质微生态、产量及果实品质的调控机制OA

In order to investigate the comprehensive effects of the strawberry and tomato intercropping pattern on crop production per-formance,substrate chemical properties,and microbial community structure,a comparative experiment was conducted between straw-berry monocropping(CK),strawberry and tomato intercropping(IT).The strawberry yield and quality,as well as substrate nutrient con-tent,were measured.High-throughput sequencing technology was employed to analyze the diversity and composition of substrate bac-terial and fungal communities,along with their correlations with key chemical factors.The results showed that,compared with CK,IT significantly increased strawberry yield(2.4%)and improved fruit quality indicators such as soluble solids and vitamin C content.Ad-ditionally,the increased tomato output contributed to a notable enhancement in the comprehensive economic benefit.IT altered the substrate nutrient status,with 5.17%increases observed only in total potassium content,while pH,organic matter,total nitrogen,total phosphorus,alkali-hydrolyzable nitrogen,available phosphorus,and available potassium contents were significantly reduced.The in-tercropping pattern decreased the Alpha diversity of bacterial communities but significantly enhanced the species richness and diversi-ty of fungal communities.At the phylum level,IT enriched bacterial phyla such as Pseudomonadota and fungal phyla such as Ascomy-cota.At the genus level,the relative abundances of beneficial genera,including Pseudoduganella and Aspergillus,were significantly increased.Correlation analysis revealed that the intercropping pattern altered the associations between dominant microbial genera and key nutrients such as nitrogen,phosphorus,and potassium in the substrate,indicating significant adaptive adjustments of the microbial community to the intercropping environment.In conclusion,the strawberry and tomato intercropping pattern can optimize the substrate microbial community structure and regulate nutrient availability,thereby achieving synergistic effects of crop yield improvement,qual-ity enhancement,and microecological optimization.This study provides a theoretical foundation for ecological intensive cultivation.