Sugen低氧诱导肺动脉高压小鼠模型中免疫细胞亚群的动态变化OA

Objective To investigate the dynamic changes of immune cell subsets in a mouse model of pulmonary hypertension(PH)induced by Sugen-hypoxia(SuHx).Methods A SuHx-PH mouse model was established through intraperitoneal injection of Sugen 5416,followed by exposure to hypoxia.Mouse lung tissues were collected prior to modeling and at 1 week(h1w),2 weeks(h2w),3 weeks(h3w),and 4 weeks(h4w)post-modeling.Flow cytometry was employed to detect the proportional alterations of immune cell subsets(including T cells,B cells,macrophages,eosinophils(EOS),neutrophils(Neu),natural killer(NK)cells,and innate lymphoid cells(ILCs)within CD45+immune cells.Results Compared with the control group,the immune cell subsets in SuHx model mice demonstrated significant temporal changes.Among these,the total CD11c+F4/80+macrophages and interstitial macrophages(IM,SIGLECF-)showed a significant decreasing tendency as the modeling time progressed;the cell levels of alveolar macrophages(AM,SIGLECF+)continuously increased(P＜0.05);EOS,ILCs,Neu,and CD4+T cells displayed a significant fluctuating pattern,with significant differences between groups(P＜0.05).Conclusions The proportions of immune cell subsets in the lung tissues of SuHx-PH mice exhibit significant temporal changes,indicating that different immune cells may play stage-specific roles in the progression of PH.This study offers a foundation for a more in-depth understanding of the immunoinflammatory mechanism of PH and the identification of potential immune intervention targets.